Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy

Juliette Humeau; Lucillia Bezu; Oliver Kepp; Laura Senovilla; Peng Liu; Guido Kroemer

doi:10.1007/978-1-0716-1217-0_15

Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy

Juliette Humeau, Lucillia Bezu, Oliver Kepp, Laura Senovilla, Peng Liu, Guido Kroemer

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

2 Citations (Scopus)

Résumé

Mitotic catastrophe is an oncosuppressive mechanism that drives cells toward senescence or death when an error occurs during mitosis. Eukaryotic cells have developed adaptive signaling pathways to cope with stress. The phosphorylation on serine 51 of the eukaryotic translation initiation factor (eIF2α) is a highly conserved event in stress responses, including the one that is activated upon treatment with mitotic catastrophe inducing agents, such as microtubular poisons or actin blockers. The protocol described herein details a method to quantify the phosphorylation of eIF2α by high-throughput immunofluorescence microscopy. This method is useful to capture the ‘integrated stress response’, which is characterized by eIF2α phosphorylation in the context of mitotic catastrophe.

langue originale	Anglais
titre	Methods in Molecular Biology
Editeur	Humana Press Inc.
Pages	217-226
Nombre de pages	10
Les DOIs	https://doi.org/10.1007/978-1-0716-1217-0_15
état	Publié - 1 janv. 2021

Série de publications

Nom	Methods in Molecular Biology
Volume	2267
ISSN (imprimé)	1064-3745
ISSN (Electronique)	1940-6029

Accès au document

10.1007/978-1-0716-1217-0_15

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Contient cette citation

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title = "Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy",

abstract = "Mitotic catastrophe is an oncosuppressive mechanism that drives cells toward senescence or death when an error occurs during mitosis. Eukaryotic cells have developed adaptive signaling pathways to cope with stress. The phosphorylation on serine 51 of the eukaryotic translation initiation factor (eIF2α) is a highly conserved event in stress responses, including the one that is activated upon treatment with mitotic catastrophe inducing agents, such as microtubular poisons or actin blockers. The protocol described herein details a method to quantify the phosphorylation of eIF2α by high-throughput immunofluorescence microscopy. This method is useful to capture the {\textquoteleft}integrated stress response{\textquoteright}, which is characterized by eIF2α phosphorylation in the context of mitotic catastrophe.",

keywords = "Endoplasmic reticulum, Immunofluorescence, Mitotic catastrophe, eIF2α",

author = "Juliette Humeau and Lucillia Bezu and Oliver Kepp and Laura Senovilla and Peng Liu and Guido Kroemer",

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Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy. / Humeau, Juliette; Bezu, Lucillia; Kepp, Oliver et al.
Methods in Molecular Biology. Humana Press Inc., 2021. p. 217-226 (Methods in Molecular Biology; Vol 2267).

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

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T1 - Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy

AU - Humeau, Juliette

AU - Bezu, Lucillia

AU - Kepp, Oliver

AU - Senovilla, Laura

AU - Liu, Peng

AU - Kroemer, Guido

PY - 2021/1/1

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AB - Mitotic catastrophe is an oncosuppressive mechanism that drives cells toward senescence or death when an error occurs during mitosis. Eukaryotic cells have developed adaptive signaling pathways to cope with stress. The phosphorylation on serine 51 of the eukaryotic translation initiation factor (eIF2α) is a highly conserved event in stress responses, including the one that is activated upon treatment with mitotic catastrophe inducing agents, such as microtubular poisons or actin blockers. The protocol described herein details a method to quantify the phosphorylation of eIF2α by high-throughput immunofluorescence microscopy. This method is useful to capture the ‘integrated stress response’, which is characterized by eIF2α phosphorylation in the context of mitotic catastrophe.

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