Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy

Juliette Humeau, Lucillia Bezu, Oliver Kepp, Laura Senovilla, Peng Liu, Guido Kroemer

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    2 Citations (Scopus)

    Résumé

    Mitotic catastrophe is an oncosuppressive mechanism that drives cells toward senescence or death when an error occurs during mitosis. Eukaryotic cells have developed adaptive signaling pathways to cope with stress. The phosphorylation on serine 51 of the eukaryotic translation initiation factor (eIF2α) is a highly conserved event in stress responses, including the one that is activated upon treatment with mitotic catastrophe inducing agents, such as microtubular poisons or actin blockers. The protocol described herein details a method to quantify the phosphorylation of eIF2α by high-throughput immunofluorescence microscopy. This method is useful to capture the ‘integrated stress response’, which is characterized by eIF2α phosphorylation in the context of mitotic catastrophe.

    langue originaleAnglais
    titreMethods in Molecular Biology
    EditeurHumana Press Inc.
    Pages217-226
    Nombre de pages10
    Les DOIs
    étatPublié - 1 janv. 2021

    Série de publications

    NomMethods in Molecular Biology
    Volume2267
    ISSN (imprimé)1064-3745
    ISSN (Electronique)1940-6029

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