Reinvestigation of DNA ligase I in axolotl and Pleurodeles development

Said Aoufouchi, Serge Hardy, Claude Prigent, Michel Philippe, Pierre Thiebaud

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

8 Citations (Scopus)

Résumé

We have recently shown that the exclusion process causing the replacement of DNA ligases II by DNA ligase I in amphibian eggs after fertilization does not occur in the case of Xenopus laevis [Hardy, S., Aoufouchi, S., Thiebaud, P., and Prigent, C., (1991) Nucleic Acids Res. 19, 701-705]. Since this result is in contradiction with the situation reported in axolotl and Pieurodeles we decided to reinvestigate such results in both species. Three different approaches have been used : (1) the substrate specificity of DNA ligase I; (2) the DNA ligase-AMP adduct reaction and (3) the immunological detection using antibodies raised against the X.laevis DNA ligase I. Our results clearly demonstrate that DNA ligase I activity is associated with a single polypeptide which is present in oocyte, unfertilized egg and embryo of both amphibians. Therefore, the hypothesis of a change in DNA ligase forms, resulting from an expression of the DNA ligase I gene in axolotl and Pieurodeles early development must be rejected. We also show that, in contradiction with published data, the unfertilized sea urchin egg contains a DNA ligase activity able to join blunt ended DNA molecules.

langue originaleAnglais
Pages (de - à)4395-4398
Nombre de pages4
journalNucleic Acids Research
Volume19
Numéro de publication16
Les DOIs
étatPublié - 25 août 1991
Modification externeOui

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