TY - JOUR
T1 - Selective expression of inhibitory Fcγ receptor by metastatic melanoma impairs tumor susceptibility to IgG-dependent cellular response
AU - Cassard, Lydie
AU - Cohen-Solal, Joel F.G.
AU - Fournier, Emilie M.
AU - Camilleri-Broët, Sophie
AU - Spatz, Alain
AU - Chouaïb, Salem
AU - Badoual, Cécile
AU - Varin, Audrey
AU - Fisson, Sylvain
AU - Duvillard, Pierre
AU - Boix, Charlotte
AU - Loncar, Shannon M.
AU - Sastre-Garau, Xavier
AU - Houghton, Alan N.
AU - Avril, Marie Françoise
AU - Gresser, Ion
AU - Fridman, Wolf H.
AU - Sautès-Fridman, Catherine
PY - 2008/12/15
Y1 - 2008/12/15
N2 - During melanoma progression, patients develop anti-tumor immunity including the production of anti-tumor antibodies. Although the strategies developed by malignant cells to escape anti-tumor cellular immunity have been extensively investigated, little is known about tumor resistance to humoral immunity. The main effect of IgG antibodies is to activate the immune response by binding to host Fc gamma receptors (FcγR) expressed by immune cells. We previously reported in a limited study that some human metastatic melanoma cells ectopically express the FcγRIIB1, an inhibitory isoform of FcγR. By analyzing a large panel of different types of human primary and metastatic solid tumors, we report herein that expression of FcγRIIB is restricted to melanoma and is acquired during tumor progression. We show that FcγRIIB expression prevents the lysis of human metastatic melanoma cells by NK cell-mediated antibody-dependent cellular cytotoxicity (ADCC) in vitro, independently of the intracytoplasmic region of FcγRIIB. Using experimental mouse models, we demonstrate that expression of FcγRIIB protects B16F0 melanoma tumors from the ADCC induced by monoclonal and polyclonal anti-tumor IgG in vivo. Thus, our results identify FcγRIIB as a marker of human metastatic melanoma that impairs the tumor susceptibility to FcγR-dependent innate effector responses.
AB - During melanoma progression, patients develop anti-tumor immunity including the production of anti-tumor antibodies. Although the strategies developed by malignant cells to escape anti-tumor cellular immunity have been extensively investigated, little is known about tumor resistance to humoral immunity. The main effect of IgG antibodies is to activate the immune response by binding to host Fc gamma receptors (FcγR) expressed by immune cells. We previously reported in a limited study that some human metastatic melanoma cells ectopically express the FcγRIIB1, an inhibitory isoform of FcγR. By analyzing a large panel of different types of human primary and metastatic solid tumors, we report herein that expression of FcγRIIB is restricted to melanoma and is acquired during tumor progression. We show that FcγRIIB expression prevents the lysis of human metastatic melanoma cells by NK cell-mediated antibody-dependent cellular cytotoxicity (ADCC) in vitro, independently of the intracytoplasmic region of FcγRIIB. Using experimental mouse models, we demonstrate that expression of FcγRIIB protects B16F0 melanoma tumors from the ADCC induced by monoclonal and polyclonal anti-tumor IgG in vivo. Thus, our results identify FcγRIIB as a marker of human metastatic melanoma that impairs the tumor susceptibility to FcγR-dependent innate effector responses.
KW - Antibodies
KW - Cytotoxicity
KW - Fc receptors
KW - Immune escape
KW - Melanoma
UR - http://www.scopus.com/inward/record.url?scp=57349198014&partnerID=8YFLogxK
U2 - 10.1002/ijc.23870
DO - 10.1002/ijc.23870
M3 - Article
C2 - 18798552
AN - SCOPUS:57349198014
SN - 0020-7136
VL - 123
SP - 2832
EP - 2839
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 12
ER -