Selective expression of inhibitory Fcγ receptor by metastatic melanoma impairs tumor susceptibility to IgG-dependent cellular response

Lydie Cassard, Joel F.G. Cohen-Solal, Emilie M. Fournier, Sophie Camilleri-Broët, Alain Spatz, Salem Chouaïb, Cécile Badoual, Audrey Varin, Sylvain Fisson, Pierre Duvillard, Charlotte Boix, Shannon M. Loncar, Xavier Sastre-Garau, Alan N. Houghton, Marie Françoise Avril, Ion Gresser, Wolf H. Fridman, Catherine Sautès-Fridman

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

29 Citations (Scopus)

Résumé

During melanoma progression, patients develop anti-tumor immunity including the production of anti-tumor antibodies. Although the strategies developed by malignant cells to escape anti-tumor cellular immunity have been extensively investigated, little is known about tumor resistance to humoral immunity. The main effect of IgG antibodies is to activate the immune response by binding to host Fc gamma receptors (FcγR) expressed by immune cells. We previously reported in a limited study that some human metastatic melanoma cells ectopically express the FcγRIIB1, an inhibitory isoform of FcγR. By analyzing a large panel of different types of human primary and metastatic solid tumors, we report herein that expression of FcγRIIB is restricted to melanoma and is acquired during tumor progression. We show that FcγRIIB expression prevents the lysis of human metastatic melanoma cells by NK cell-mediated antibody-dependent cellular cytotoxicity (ADCC) in vitro, independently of the intracytoplasmic region of FcγRIIB. Using experimental mouse models, we demonstrate that expression of FcγRIIB protects B16F0 melanoma tumors from the ADCC induced by monoclonal and polyclonal anti-tumor IgG in vivo. Thus, our results identify FcγRIIB as a marker of human metastatic melanoma that impairs the tumor susceptibility to FcγR-dependent innate effector responses.

langue originaleAnglais
Pages (de - à)2832-2839
Nombre de pages8
journalInternational Journal of Cancer
Volume123
Numéro de publication12
Les DOIs
étatPublié - 15 déc. 2008
Modification externeOui

Contient cette citation