Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death

Lenka Kasikova; Iva Truxova; Isabelle Cremer; Catherine Sautes-Fridman; Oliver Kepp; Guido Kroemer; Radek Spisek; Jitka Fucikova

doi:10.1016/bs.mie.2019.05.025

Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death

Lenka Kasikova, Iva Truxova, Isabelle Cremer, Catherine Sautes-Fridman, Oliver Kepp, Guido Kroemer, Radek Spisek, Jitka Fucikova

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

3 Citations (Scopus)

Résumé

Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.

langue originale	Anglais
titre	Tumor Immunology and Immunotherapy - Cellular Methods Part B
rédacteurs en chef	Lorenzo Galluzzi, Nils-Petter Rudqvist
Editeur	Academic Press Inc.
Pages	15-25
Nombre de pages	11
ISBN (imprimé)	9780128186756
Les DOIs	https://doi.org/10.1016/bs.mie.2019.05.025
état	Publié - 1 janv. 2020

Série de publications

Nom	Methods in Enzymology
Volume	632
ISSN (imprimé)	0076-6879
ISSN (Electronique)	1557-7988

Accès au document

10.1016/bs.mie.2019.05.025

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Contient cette citation

Kasikova, L., Truxova, I., Cremer, I., Sautes-Fridman, C., Kepp, O., Kroemer, G., Spisek, R., & Fucikova, J. (2020). Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death. Dans L. Galluzzi, & N.-P. Rudqvist (eds.), Tumor Immunology and Immunotherapy - Cellular Methods Part B (p. 15-25). (Methods in Enzymology; Vol 632). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2019.05.025

Kasikova, Lenka ; Truxova, Iva ; Cremer, Isabelle et al. / Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death. Tumor Immunology and Immunotherapy - Cellular Methods Part B. Editeur / Lorenzo Galluzzi ; Nils-Petter Rudqvist. Academic Press Inc., 2020. p. 15-25 (Methods in Enzymology).

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title = "Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death",

abstract = "Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.",

keywords = "Calreticulin, Cancer prognosis, Flow cytometry, Immunogenic cell death, Immunohistochemistry",

author = "Lenka Kasikova and Iva Truxova and Isabelle Cremer and Catherine Sautes-Fridman and Oliver Kepp and Guido Kroemer and Radek Spisek and Jitka Fucikova",

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doi = "10.1016/bs.mie.2019.05.025",

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Kasikova, L, Truxova, I, Cremer, I, Sautes-Fridman, C, Kepp, O, Kroemer, G, Spisek, R & Fucikova, J 2020, Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death. Dans L Galluzzi & N-P Rudqvist (eds), Tumor Immunology and Immunotherapy - Cellular Methods Part B. Methods in Enzymology, VOL. 632, Academic Press Inc., p. 15-25. https://doi.org/10.1016/bs.mie.2019.05.025

Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death. / Kasikova, Lenka; Truxova, Iva; Cremer, Isabelle et al.
Tumor Immunology and Immunotherapy - Cellular Methods Part B. Ed. / Lorenzo Galluzzi; Nils-Petter Rudqvist. Academic Press Inc., 2020. p. 15-25 (Methods in Enzymology; Vol 632).

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

TY - CHAP

T1 - Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death

AU - Kasikova, Lenka

AU - Truxova, Iva

AU - Cremer, Isabelle

AU - Sautes-Fridman, Catherine

AU - Kepp, Oliver

AU - Kroemer, Guido

AU - Spisek, Radek

AU - Fucikova, Jitka

PY - 2020/1/1

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N2 - Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.

AB - Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.

KW - Calreticulin

KW - Cancer prognosis

KW - Flow cytometry

KW - Immunogenic cell death

KW - Immunohistochemistry

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BT - Tumor Immunology and Immunotherapy - Cellular Methods Part B

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Kasikova L, Truxova I, Cremer I, Sautes-Fridman C, Kepp O, Kroemer G et al. Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death. Dans Galluzzi L, Rudqvist NP, rédacteurs en chef, Tumor Immunology and Immunotherapy - Cellular Methods Part B. Academic Press Inc. 2020. p. 15-25. (Methods in Enzymology). doi: 10.1016/bs.mie.2019.05.025