Spectral Flow Cytometry Analysis of Resident Tissue Macrophages

Wan Ting Kong, Mathilde Bied, Florent Ginhoux

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    1 Citation (Scopus)

    Résumé

    Spectral flow cytometry improves flow cytometry panels by resolving the full emission spectra of individual fluorophores, allowing greater flexibility to incorporate more fluorochromes when designing multicolor panels. Additionally, the spectral approach captures the autofluorescence of a sample or cell population (e.g., macrophages, which are highly autofluorescent) that can be considered during unmixing for improved downstream analyses. As the increased complexity of macrophage heterogeneity unravels in the scientific community, it is crucial to obtain high-dimensional data at the single-cell level to resolve these populations.

    langue originaleAnglais
    titreMethods in Molecular Biology
    EditeurHumana Press Inc.
    Pages269-280
    Nombre de pages12
    Les DOIs
    étatPublié - 1 janv. 2024

    Série de publications

    NomMethods in Molecular Biology
    Volume2713
    ISSN (imprimé)1064-3745
    ISSN (Electronique)1940-6029

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