Structural organization of the hCTLA-1 gene encoding human granzyme B

Patrick Haddad; Marie Véronique Clément; Olivier Bernard; Christian Jacques Larsen; Laurent Degos; Marilyne Sasportes; Danièle Mathieu-Mahul

doi:10.1016/0378-1119(90)90311-E

Structural organization of the hCTLA-1 gene encoding human granzyme B

Patrick Haddad, Marie Véronique Clément, Olivier Bernard, Christian Jacques Larsen, Laurent Degos, Marilyne Sasportes, Danièle Mathieu-Mahul

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

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Résumé

Cytotoxic T lymphocytes (CTLs) and natural killer/lymphokine-activated cells produce granzymes, a family of serine esterase proteins located in cytoplasmic granules. These might be involved in different cytotoxic pathways. We report the structural organization of the human gene encoding granzyme B (hCTLA-1). A 4.75-kb genomic DNA fragment containing all the sequences of granzyme B-encoding cDNA clones has been sequenced. The gene is composed of five exons and four introns. A comparison with the genomic organization of murine CCP1/CTLA-1 showed very similar structure and a 76% nucleotide homology in the coding sequences. This suggests that both genes may have a common ancestor. No typical regulatory element was detected in the 1160 bp upstream from the ATG start codon. The detection of a second locus related to hCTLA-1 is also described.

langue originale	Anglais
Pages (de - à)	265-271
Nombre de pages	7
journal	Gene
Volume	87
Numéro de publication	2
Les DOIs	https://doi.org/10.1016/0378-1119(90)90311-E
état	Publié - 15 mars 1990

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10.1016/0378-1119(90)90311-E

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@article{c13a27b8d5d74aceb8602f72c79260ad,

title = "Structural organization of the hCTLA-1 gene encoding human granzyme B",

abstract = "Cytotoxic T lymphocytes (CTLs) and natural killer/lymphokine-activated cells produce granzymes, a family of serine esterase proteins located in cytoplasmic granules. These might be involved in different cytotoxic pathways. We report the structural organization of the human gene encoding granzyme B (hCTLA-1). A 4.75-kb genomic DNA fragment containing all the sequences of granzyme B-encoding cDNA clones has been sequenced. The gene is composed of five exons and four introns. A comparison with the genomic organization of murine CCP1/CTLA-1 showed very similar structure and a 76% nucleotide homology in the coding sequences. This suggests that both genes may have a common ancestor. No typical regulatory element was detected in the 1160 bp upstream from the ATG start codon. The detection of a second locus related to hCTLA-1 is also described.",

keywords = "Recombinant DNA, T lymphocyte, cloning, cytotoxicity, perforin, phage λ vector, plasmid, serine esterase",

author = "Patrick Haddad and Cl{\'e}ment, {Marie V{\'e}ronique} and Olivier Bernard and Larsen, {Christian Jacques} and Laurent Degos and Marilyne Sasportes and Dani{\`e}le Mathieu-Mahul",

note = "Funding Information: We wish to express our gratitude to Zhu Chen who made available the genomic library, to Pascale Paul, Annie Soulit, Jacqueline Guillet and Martine Mauchauff6 for their cooperation and stimulating discussions and to Bernard Boursin and Jacqueline Boisse for preparing photographs. This work was supported by a grant from the Association pour la Recherche contre le Cancer (ARC).",

year = "1990",

month = mar,

day = "15",

doi = "10.1016/0378-1119(90)90311-E",

language = "English",

volume = "87",

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T1 - Structural organization of the hCTLA-1 gene encoding human granzyme B

AU - Haddad, Patrick

AU - Clément, Marie Véronique

AU - Bernard, Olivier

AU - Larsen, Christian Jacques

AU - Degos, Laurent

AU - Sasportes, Marilyne

AU - Mathieu-Mahul, Danièle

N1 - Funding Information: We wish to express our gratitude to Zhu Chen who made available the genomic library, to Pascale Paul, Annie Soulit, Jacqueline Guillet and Martine Mauchauff6 for their cooperation and stimulating discussions and to Bernard Boursin and Jacqueline Boisse for preparing photographs. This work was supported by a grant from the Association pour la Recherche contre le Cancer (ARC).

PY - 1990/3/15

Y1 - 1990/3/15

N2 - Cytotoxic T lymphocytes (CTLs) and natural killer/lymphokine-activated cells produce granzymes, a family of serine esterase proteins located in cytoplasmic granules. These might be involved in different cytotoxic pathways. We report the structural organization of the human gene encoding granzyme B (hCTLA-1). A 4.75-kb genomic DNA fragment containing all the sequences of granzyme B-encoding cDNA clones has been sequenced. The gene is composed of five exons and four introns. A comparison with the genomic organization of murine CCP1/CTLA-1 showed very similar structure and a 76% nucleotide homology in the coding sequences. This suggests that both genes may have a common ancestor. No typical regulatory element was detected in the 1160 bp upstream from the ATG start codon. The detection of a second locus related to hCTLA-1 is also described.

AB - Cytotoxic T lymphocytes (CTLs) and natural killer/lymphokine-activated cells produce granzymes, a family of serine esterase proteins located in cytoplasmic granules. These might be involved in different cytotoxic pathways. We report the structural organization of the human gene encoding granzyme B (hCTLA-1). A 4.75-kb genomic DNA fragment containing all the sequences of granzyme B-encoding cDNA clones has been sequenced. The gene is composed of five exons and four introns. A comparison with the genomic organization of murine CCP1/CTLA-1 showed very similar structure and a 76% nucleotide homology in the coding sequences. This suggests that both genes may have a common ancestor. No typical regulatory element was detected in the 1160 bp upstream from the ATG start codon. The detection of a second locus related to hCTLA-1 is also described.

KW - Recombinant DNA

KW - T lymphocyte

KW - cloning

KW - cytotoxicity

KW - perforin

KW - phage λ vector

KW - plasmid

KW - serine esterase

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SN - 0378-1119

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