@inbook{6719ccf372df40ec9f3609c00a808052,
title = "Studying intracellular trafficking pathways with probabilistic density maps",
abstract = "The compartmentalization of cellular functions in complex membranous organelles is a key feature of eukaryotic cells. To cope with the enormous complexity of trafficking pathways that connect these compartments, new approaches need to be considered and introduced into the field of cell biology. We exploit the advantages of the {"}micropatterning technique,{"} which is to bring cells to adopt a highly reproducible shape, and probabilistic density mapping, which quantifies spatial organization of trafficking compartments, to study regulatory mechanisms of intracellular trafficking. Here, we provide a protocol to analyze and quantify alterations in trafficking compartments upon cellular manipulation. We demonstrate how this approach can be employed to study the regulation of Rab6-labeled transport carriers by the cytoskeleton.",
keywords = "Density mapping, Kernel density estimation, Micropatterning, Quantitative cell biology, Rab GTPases, Rab6",
author = "Kristine Schauer and Tarn Duong and Gomes-Santos, {Carina S.} and Bruno Goud",
note = "Funding Information: We thank Laura Picas and Lena Oesterlin for critical reading of the chapter. K. S. received funding from the Fondation pour la Recherche M{\'e}dicale en France and Association pour la Recherche sur le Cancer. T. D. received funding from the program “Investissements d{\textquoteright}avenir” ANR-10-IAIHU-06. C. S. G. S. is supported by the Marie Curie Fellowship PIEF-GA-2011-299756. This project was further supported by grants from Agence Nationale de la Recherche (#2010 BLAN 122902), the Centre National de la Recherche Scientifique, and Institut Curie. ",
year = "2013",
month = jan,
day = "1",
doi = "10.1016/B978-0-12-417164-0.00020-3",
language = "English",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "325--343",
booktitle = "Methods in Cell Biology",
}