Targeted Analysis of Glycerophospholipids and Mono-, Di-, or Tri-Acylglycerides in Liver Cancer

Hui Chen, Sylvère Durand, Mélanie Bourgin, Flavia Lambertucci, Omar Motiño, Léa Montégut, Sijing Li, Uxía Nogueira-Recalde, Gerasimos Anagnostopoulos, Maria Chiara Maiuri, Guido Kroemer, Isabelle Martins

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    Résumé

    The metabolic rearrangements of hepatic metabolism associated with liver cancer are still incompletely understood. There is an ongoing need to identify novel and more efficient diagnostic biomarkers and therapeutic targets based on the metabolic mechanisms of these diseases. In comparison to traditional diagnostic biomarkers, metabolomics is a comprehensive technique for discovering chemical signatures for liver cancer screening, prediction, and earlier diagnosis. Lipids are a large and diverse group of complex biomolecules that are at the heart of liver physiology and play an important role in the development and progression of cancer. In this chapter, we described two detailed protocols for targeted lipids analysis: glycerophospholipids and mono, di, tri-acylglycerides, both by Flow Injection Analysis (FIA) HPLC coupled to a SelexIon/QTRAP 6500+ system. These approaches provide a targeted lipidomic metabolomic signature of dissimilar metabolic disorders affecting liver cancers.

    langue originaleAnglais
    titreMethods in Molecular Biology
    EditeurHumana Press Inc.
    Pages189-198
    Nombre de pages10
    Les DOIs
    étatPublié - 1 janv. 2024

    Série de publications

    NomMethods in Molecular Biology
    Volume2769
    ISSN (imprimé)1064-3745
    ISSN (Electronique)1940-6029

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