Résumé
A cDNA clone coding for DNA ligase I (LigI) was isolated from a Xenopus laevis oocyte cDNA library. The 3766-bp sequence showed a putative ORF capable of encoding a 1070-amino-acid protein whose overall identity with two mammalian sequences is 63%. This identity, however, rises to 72.5% in the C-terminal portion of the protein that contains the active site. Expression of the cDNA in a prokaryotic system produces a protein that is immunologically identical to LigI and can be adenylated. The 180-kDa size of the recombinant protein is similar to the LigI detected in oocyte. Northern blot analysis of ovary and embryo RNAs revealed the expression of two (4.1 and 6 kb) LigI transcripts.
langue originale | Anglais |
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Pages (de - à) | 273-277 |
Nombre de pages | 5 |
journal | Gene |
Volume | 172 |
Numéro de publication | 2 |
Les DOIs | |
état | Publié - 26 juin 1996 |
Modification externe | Oui |