@inbook{c6e2b267124945089e9464b3699cdfab,
title = "The intracellular metabolome of starving cells",
abstract = "Fasting induces vast metabolic adaptations on the cellular level and leads to an organism-wide induction of autophagy. Autophagic degradation subserves resource recycling and facilitates the maintenance of energetic homeostasis. Mass spectrometry offers the possibility to assess changes in the metabolome that occur in conditions of nutrient deprivation and to profile such adaptations. Here we describe a detailed workflow for the targeted quantitation and untargeted profiling of metabolites that can be used to assess the intracellular metabolome of starving cells. Moreover, we outline a workflow for the use of non-radioactive isotope labeled metabolites. Altogether, we show that mass spectrometry is a powerful tool for monitoring metabolic changes in conditions of fasting.",
keywords = "Autophagy, Chromatography, Mass spectrometry, Metabolites",
author = "Sylv{\`e}re Durand and Claudia Grajeda-Iglesias and Fanny Aprahamian and Nitharsshini Nirmalathasan and Oliver Kepp and Guido Kroemer",
note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jan,
day = "1",
doi = "10.1016/bs.mcb.2021.04.001",
language = "English",
isbn = "9780128235447",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "137--156",
editor = "Oliver Kepp and Lorenzo Galluzzi",
booktitle = "Monitoring vesicular trafficking in cellular responses to stress - Part A",
}