TY - JOUR
T1 - The lifespan and kinetics of human dendritic cell subsets and their precursors in health and inflammation
AU - Lubin, Ruth
AU - Patel, Amit A.
AU - Mackerodt, Jonas
AU - Zhang, Yan
AU - Gvili, Rotem
AU - Mulder, Kevin
AU - Dutertre, Charles Antoine
AU - Jalali, Parinaaz
AU - Glanville, James R.W.
AU - Hazan, Idit
AU - Sridharan, Nikhila
AU - Rivkin, Gurion
AU - Akarca, Ayse
AU - Marafioti, Teresa
AU - Gilroy, Derek W.
AU - Kandel, Leonid
AU - Mildner, Alexander
AU - Wilensky, Asaf
AU - Asquith, Becca
AU - Ginhoux, Florent
AU - Macallan, Derek
AU - Yona, Simon
N1 - Publisher Copyright:
© 2024 Lubin et al.
PY - 2024/11/4
Y1 - 2024/11/4
N2 - Dendritic cells (DC) are specialized mononuclear phagocytes that link innate and adaptive immunity. They comprise two principal subsets: plasmacytoid DC (pDC) and conventional DC (cDC). Understanding the generation, differentiation, and migration of cDC is critical for immune homeostasis. Through human in vivo deuterium-glucose labeling, we observed the rapid appearance of AXL+ Siglec6+ DC (ASDC) in the bloodstream. ASDC circulate for ∼2.16 days, while cDC1 and DC2 circulate for ∼1.32 and ∼2.20 days, respectively, upon release from the bone marrow. Interestingly, DC3, a cDC subset that shares several similarities with monocytes, exhibits a labeling profile closely resembling that of DC2. In a human in vivo model of cutaneous inflammation, ASDC were recruited to the inflammatory site, displaying a distinctive effector signature. Taken together, these results quantify the ephemeral circulating lifespan of human cDC and propose functions of cDC and their precursors that are rapidly recruited to sites of inflammation.
AB - Dendritic cells (DC) are specialized mononuclear phagocytes that link innate and adaptive immunity. They comprise two principal subsets: plasmacytoid DC (pDC) and conventional DC (cDC). Understanding the generation, differentiation, and migration of cDC is critical for immune homeostasis. Through human in vivo deuterium-glucose labeling, we observed the rapid appearance of AXL+ Siglec6+ DC (ASDC) in the bloodstream. ASDC circulate for ∼2.16 days, while cDC1 and DC2 circulate for ∼1.32 and ∼2.20 days, respectively, upon release from the bone marrow. Interestingly, DC3, a cDC subset that shares several similarities with monocytes, exhibits a labeling profile closely resembling that of DC2. In a human in vivo model of cutaneous inflammation, ASDC were recruited to the inflammatory site, displaying a distinctive effector signature. Taken together, these results quantify the ephemeral circulating lifespan of human cDC and propose functions of cDC and their precursors that are rapidly recruited to sites of inflammation.
UR - http://www.scopus.com/inward/record.url?scp=85206960182&partnerID=8YFLogxK
U2 - 10.1084/jem.20220867
DO - 10.1084/jem.20220867
M3 - Article
C2 - 39417994
AN - SCOPUS:85206960182
SN - 0022-1007
VL - 221
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 11
ER -