The neuroprotective steroid progesterone promotes mitochondrial uncoupling, reduces cytosolic calcium and augments stress resistance in yeast cells

Slaven Stekovic, Christoph Ruckenstuhl, Philipp Royer, Christof Winkler-Hermaden, Didac Carmona-Gutierrez, Kai Uwe Fröhlich, Guido Kroemer, Frank Madeo

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    10 Citations (Scopus)

    Résumé

    The steroid hormone progesterone is not only a crucial sex hormone, but also serves as a neurosteroid, thus playing an important role in brain function. Epidemiological data suggest that progesterone improves the recovery of patients after traumatic brain injury. Brain injuries are often connected to elevated calcium spikes, reactive oxygen species (ROS) and programmed cell death affecting neurons. Here, we establish a yeast model to study progesterone-mediated cytoprotection. External supply of progesterone protected yeast cells from apoptosis-inducing stress stimuli and resulted in elevated mitochondrial oxygen uptake accompanied by a drop in ROS generation and ATP levels during chronological aging. In addition, cellular Ca2+ concentrations were reduced upon progesterone treatment, and this effect occurred independently of known Ca2+ transporters and mitochondrial respiration. All effects were also independent of Dap1, the yeast orthologue of the progesterone receptor. Altogether, our observations provide new insights into the cytoprotective effects of progesterone.

    langue originaleAnglais
    Pages (de - à)191-199
    Nombre de pages9
    journalMicrobial Cell
    Volume4
    Numéro de publication6
    Les DOIs
    étatPublié - 1 juin 2017

    Contient cette citation