TY - JOUR
T1 - Tissue-resident macrophages self-maintain locally throughout adult life with minimal contribution from circulating monocytes
AU - Hashimoto, Daigo
AU - Chow, Andrew
AU - Noizat, Clara
AU - Teo, Pearline
AU - Beasley, Mary Beth
AU - Leboeuf, Marylene
AU - Becker, Christian D.
AU - See, Peter
AU - Price, Jeremy
AU - Lucas, Daniel
AU - Greter, Melanie
AU - Mortha, Arthur
AU - Boyer, Scott W.
AU - Forsberg, E. Camilla
AU - Tanaka, Masato
AU - van Rooijen, Nico
AU - García-Sastre, Adolfo
AU - Stanley, E. Richard
AU - Ginhoux, Florent
AU - Frenette, Paul S.
AU - Merad, Miriam
N1 - Funding Information:
M.M. is supported by National Institutes of Health (NIH) grants CA154947A, AI10008, and AI089987. P.S.F. is supported by NIH grants HL116340, DK056638, HL097700, and HL069438. A.C. is supported by a predoctoral fellowship from the National Heart, Lung, and Blood Institute (5F30HL099028). S.W.B. is supported by predoctoral NIH training grant 2T32GM008646 to the University of California-Santa Cruz; E.C.F. is supported by a California Institute for Regenerative Medicine New Faculty Award.
PY - 2013/4/18
Y1 - 2013/4/18
N2 - Despite accumulating evidence suggesting local self-maintenance of tissue macrophages in the steady state, the dogma remains that tissue macrophages derive from monocytes. Using parabiosis and fate-mapping approaches, we confirmed that monocytes do not show significant contribution to tissue macrophages in the steady state. Similarly, we found that after depletion of lung macrophages, the majority of repopulation occurred by stochastic cellular proliferation in situ in a macrophage colony-stimulating factor (M-Csf)- and granulocyte macrophage (GM)-CSF-dependent manner but independently of interleukin-4. We also found that after bone marrow transplantation, host macrophages retained the capacity to expand when the development of donor macrophages was compromised. Expansion of host macrophages was functional and prevented the development of alveolar proteinosis in mice transplanted with GM-Csf-receptor-deficient progenitors. Collectively, these results indicate that tissue-resident macrophages and circulating monocytes should be classified as mononuclear phagocyte lineages that are independently maintained in the steady state.
AB - Despite accumulating evidence suggesting local self-maintenance of tissue macrophages in the steady state, the dogma remains that tissue macrophages derive from monocytes. Using parabiosis and fate-mapping approaches, we confirmed that monocytes do not show significant contribution to tissue macrophages in the steady state. Similarly, we found that after depletion of lung macrophages, the majority of repopulation occurred by stochastic cellular proliferation in situ in a macrophage colony-stimulating factor (M-Csf)- and granulocyte macrophage (GM)-CSF-dependent manner but independently of interleukin-4. We also found that after bone marrow transplantation, host macrophages retained the capacity to expand when the development of donor macrophages was compromised. Expansion of host macrophages was functional and prevented the development of alveolar proteinosis in mice transplanted with GM-Csf-receptor-deficient progenitors. Collectively, these results indicate that tissue-resident macrophages and circulating monocytes should be classified as mononuclear phagocyte lineages that are independently maintained in the steady state.
UR - http://www.scopus.com/inward/record.url?scp=84876775203&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2013.04.004
DO - 10.1016/j.immuni.2013.04.004
M3 - Article
C2 - 23601688
AN - SCOPUS:84876775203
SN - 1074-7613
VL - 38
SP - 792
EP - 804
JO - Immunity
JF - Immunity
IS - 4
ER -