Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming

Joseph Wolfers; Anne Lozier; Graça Raposo; Armelle Regnault; Clotilde Théry; Carole Masurier; Caroline Flament; Stéphanie Pouzieux; Florence Faure; Thomas Tursz; Eric Angevin; Sebastian Amigorena; Laurence Zitvogel

doi:10.1038/85438

Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming

Joseph Wolfers, Anne Lozier, Graça Raposo, Armelle Regnault, Clotilde Théry, Carole Masurier, Caroline Flament, Stéphanie Pouzieux, Florence Faure, Thomas Tursz, Eric Angevin, Sebastian Amigorena, Laurence Zitvogel

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

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Résumé

The initiation of T-cell-mediated antitumor immune responses requires the uptake and processing of tumor antigens by dendritic cells and their presentation on MHC-l molecules. Here we show in a human in vitro model system that exosomes, a population of small membrane vesicles secreted by living tumor cells, contain and transfer tumor antigens to dendritic cells. After mouse tumor exosome uptake, dendritic cells induce potent CD8⁺ T-cell-dependent antitumor effects on syngeneic and allogeneic established mouse tumors. Therefore, exosomes represent a novel source of tumor-rejection antigens for T-cell cross priming, relevant for immunointerventions.

langue originale	Anglais
Pages (de - à)	297-303
Nombre de pages	7
journal	Nature Medicine
Volume	7
Numéro de publication	3
Les DOIs	https://doi.org/10.1038/85438
état	Publié - 29 mars 2001

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10.1038/85438

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@article{930e14884432411c8bd243f232d91979,

title = "Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming",

abstract = "The initiation of T-cell-mediated antitumor immune responses requires the uptake and processing of tumor antigens by dendritic cells and their presentation on MHC-l molecules. Here we show in a human in vitro model system that exosomes, a population of small membrane vesicles secreted by living tumor cells, contain and transfer tumor antigens to dendritic cells. After mouse tumor exosome uptake, dendritic cells induce potent CD8+ T-cell-dependent antitumor effects on syngeneic and allogeneic established mouse tumors. Therefore, exosomes represent a novel source of tumor-rejection antigens for T-cell cross priming, relevant for immunointerventions.",

author = "Joseph Wolfers and Anne Lozier and Gra{\c c}a Raposo and Armelle Regnault and Clotilde Th{\'e}ry and Carole Masurier and Caroline Flament and St{\'e}phanie Pouzieux and Florence Faure and Thomas Tursz and Eric Angevin and Sebastian Amigorena and Laurence Zitvogel",

note = "Funding Information: Acknowledgments We thank F. Andr{\'e}, A. Caignard and C. Bonnerot for helpful discussions; M.-F. Avril for providing us with melanoma patient-derived tumor material; A. Le Cesne for blood samples; S. Koscielny for statistical analysis; and the staff of the animal facility for animal care and handling. This work was supported by the Ligue Fran{\c c}aise de Lutte Contre le Cancer {\textquoteleft}Axe Immunologie des Tumeurs{\textquoteright}, INSERM, CNRS, APCells SA and Inc., GEFLUC and Association pour la Recherche Contre Le Cancer ARC. J.W. was supported by Ligue Nationale de Lutte Contre le Cancer, A.L. by ARC.",

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AU - Wolfers, Joseph

AU - Lozier, Anne

AU - Raposo, Graça

AU - Regnault, Armelle

AU - Théry, Clotilde

AU - Masurier, Carole

AU - Flament, Caroline

AU - Pouzieux, Stéphanie

AU - Faure, Florence

AU - Tursz, Thomas

AU - Angevin, Eric

AU - Amigorena, Sebastian

AU - Zitvogel, Laurence

N1 - Funding Information: Acknowledgments We thank F. André, A. Caignard and C. Bonnerot for helpful discussions; M.-F. Avril for providing us with melanoma patient-derived tumor material; A. Le Cesne for blood samples; S. Koscielny for statistical analysis; and the staff of the animal facility for animal care and handling. This work was supported by the Ligue Française de Lutte Contre le Cancer ‘Axe Immunologie des Tumeurs’, INSERM, CNRS, APCells SA and Inc., GEFLUC and Association pour la Recherche Contre Le Cancer ARC. J.W. was supported by Ligue Nationale de Lutte Contre le Cancer, A.L. by ARC.

PY - 2001/3/29

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N2 - The initiation of T-cell-mediated antitumor immune responses requires the uptake and processing of tumor antigens by dendritic cells and their presentation on MHC-l molecules. Here we show in a human in vitro model system that exosomes, a population of small membrane vesicles secreted by living tumor cells, contain and transfer tumor antigens to dendritic cells. After mouse tumor exosome uptake, dendritic cells induce potent CD8+ T-cell-dependent antitumor effects on syngeneic and allogeneic established mouse tumors. Therefore, exosomes represent a novel source of tumor-rejection antigens for T-cell cross priming, relevant for immunointerventions.

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