Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches

Svetoslav Chakarov; Hwee Ying Lim; Leonard Tan; Sheau Yng Lim; Peter See; Josephine Lum; Xiao Meng Zhang; Shihui Foo; Satoshi Nakamizo; Kaibo Duan; Wan Ting Kong; Rebecca Gentek; Akhila Balachander; Daniel Carbajo; Camille Bleriot; Benoit Malleret; John Kit Chung Tam; Sonia Baig; Muhammad Shabeer; Sue Anne Ee Shiow Toh; Andreas Schlitzer; Anis Larbi; Thomas Marichal; Bernard Malissen; Jinmiao Chen; Michael Poidinger; Kenji Kabashima; Marc Bajenoff; Lai Guan Ng; Veronique Angeli; Florent Ginhoux

doi:10.1126/science.aau0964

Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches

Svetoslav Chakarov, Hwee Ying Lim, Leonard Tan, Sheau Yng Lim, Peter See, Josephine Lum, Xiao Meng Zhang, Shihui Foo, Satoshi Nakamizo, Kaibo Duan, Wan Ting Kong, Rebecca Gentek, Akhila Balachander, Daniel Carbajo, Camille Bleriot, Benoit Malleret, John Kit Chung Tam, Sonia Baig, Muhammad Shabeer, Sue Anne Ee Shiow TohAndreas Schlitzer, Anis Larbi, Thomas Marichal, Bernard Malissen, Jinmiao Chen, Michael Poidinger, Kenji Kabashima, Marc Bajenoff, Lai Guan Ng, Veronique Angeli, Florent Ginhoux

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

617 Citations (Scopus)

Résumé

Macrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation, and various pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within the tissue parenchyma remain poorly defined. Here we studied IMs from murine lung, fat, heart, and dermis. We identified two independent IM subpopulations that are conserved across tissues: Lyve1 ^lo MHCII ^hi CX3CR1 ^hi (Lyve1 ^lo MHCII ^hi ) and Lyve1 ^hi MHCII ^lo CX3CR1 ^lo (Lyve1 ^hi MHCII ^lo ) monocyte-derived IMs, with distinct gene expression profiles, phenotypes, functions, and localizations. Using a new mouse model of inducible macrophage depletion (Slco2b1 ^flox/DTR ), we found that the absence of Lyve1 ^hi MHCII ^lo IMs exacerbated experimental lung fibrosis. Thus, we demonstrate that two independent populations of IMs coexist across tissues and exhibit conserved niche-dependent functional programming.

langue originale	Anglais
Numéro d'article	eaau0964
journal	Science
Volume	363
Numéro de publication	6432
Les DOIs	https://doi.org/10.1126/science.aau0964
état	Publié - 1 janv. 2019
Modification externe	Oui

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10.1126/science.aau0964

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Chakarov, S., Lim, H. Y., Tan, L., Lim, S. Y., See, P., Lum, J., Zhang, X. M., Foo, S., Nakamizo, S., Duan, K., Kong, W. T., Gentek, R., Balachander, A., Carbajo, D., Bleriot, C., Malleret, B., Tam, J. K. C., Baig, S., Shabeer, M., ... Ginhoux, F. (2019). Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches. Science, 363(6432), Article eaau0964. https://doi.org/10.1126/science.aau0964

@article{abc207a4e468400cb87e4fd7a0828a19,

title = "Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches",

abstract = " Macrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation, and various pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within the tissue parenchyma remain poorly defined. Here we studied IMs from murine lung, fat, heart, and dermis. We identified two independent IM subpopulations that are conserved across tissues: Lyve1 lo MHCII hi CX3CR1 hi (Lyve1 lo MHCII hi ) and Lyve1 hi MHCII lo CX3CR1 lo (Lyve1 hi MHCII lo ) monocyte-derived IMs, with distinct gene expression profiles, phenotypes, functions, and localizations. Using a new mouse model of inducible macrophage depletion (Slco2b1 flox/DTR ), we found that the absence of Lyve1 hi MHCII lo IMs exacerbated experimental lung fibrosis. Thus, we demonstrate that two independent populations of IMs coexist across tissues and exhibit conserved niche-dependent functional programming.",

author = "Svetoslav Chakarov and Lim, {Hwee Ying} and Leonard Tan and Lim, {Sheau Yng} and Peter See and Josephine Lum and Zhang, {Xiao Meng} and Shihui Foo and Satoshi Nakamizo and Kaibo Duan and Kong, {Wan Ting} and Rebecca Gentek and Akhila Balachander and Daniel Carbajo and Camille Bleriot and Benoit Malleret and Tam, {John Kit Chung} and Sonia Baig and Muhammad Shabeer and Toh, {Sue Anne Ee Shiow} and Andreas Schlitzer and Anis Larbi and Thomas Marichal and Bernard Malissen and Jinmiao Chen and Michael Poidinger and Kenji Kabashima and Marc Bajenoff and Ng, {Lai Guan} and Veronique Angeli and Florent Ginhoux",

note = "Publisher Copyright: 2017 {\textcopyright} The Authors.",

year = "2019",

month = jan,

day = "1",

doi = "10.1126/science.aau0964",

language = "English",

volume = "363",

journal = "Science",

issn = "0036-8075",

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Chakarov, S, Lim, HY, Tan, L, Lim, SY, See, P, Lum, J, Zhang, XM, Foo, S, Nakamizo, S, Duan, K, Kong, WT, Gentek, R, Balachander, A, Carbajo, D, Bleriot, C, Malleret, B, Tam, JKC, Baig, S, Shabeer, M, Toh, SAES, Schlitzer, A, Larbi, A, Marichal, T, Malissen, B, Chen, J, Poidinger, M, Kabashima, K, Bajenoff, M, Ng, LG, Angeli, V & Ginhoux, F 2019, 'Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches', Science, VOL. 363, Numéro 6432, eaau0964. https://doi.org/10.1126/science.aau0964

TY - JOUR

T1 - Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches

AU - Chakarov, Svetoslav

AU - Lim, Hwee Ying

AU - Tan, Leonard

AU - Lim, Sheau Yng

AU - See, Peter

AU - Lum, Josephine

AU - Zhang, Xiao Meng

AU - Foo, Shihui

AU - Nakamizo, Satoshi

AU - Duan, Kaibo

AU - Kong, Wan Ting

AU - Gentek, Rebecca

AU - Balachander, Akhila

AU - Carbajo, Daniel

AU - Bleriot, Camille

AU - Malleret, Benoit

AU - Tam, John Kit Chung

AU - Baig, Sonia

AU - Shabeer, Muhammad

AU - Toh, Sue Anne Ee Shiow

AU - Schlitzer, Andreas

AU - Larbi, Anis

AU - Marichal, Thomas

AU - Malissen, Bernard

AU - Chen, Jinmiao

AU - Poidinger, Michael

AU - Kabashima, Kenji

AU - Bajenoff, Marc

AU - Ng, Lai Guan

AU - Angeli, Veronique

AU - Ginhoux, Florent

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Macrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation, and various pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within the tissue parenchyma remain poorly defined. Here we studied IMs from murine lung, fat, heart, and dermis. We identified two independent IM subpopulations that are conserved across tissues: Lyve1 lo MHCII hi CX3CR1 hi (Lyve1 lo MHCII hi ) and Lyve1 hi MHCII lo CX3CR1 lo (Lyve1 hi MHCII lo ) monocyte-derived IMs, with distinct gene expression profiles, phenotypes, functions, and localizations. Using a new mouse model of inducible macrophage depletion (Slco2b1 flox/DTR ), we found that the absence of Lyve1 hi MHCII lo IMs exacerbated experimental lung fibrosis. Thus, we demonstrate that two independent populations of IMs coexist across tissues and exhibit conserved niche-dependent functional programming.

AB - Macrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation, and various pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within the tissue parenchyma remain poorly defined. Here we studied IMs from murine lung, fat, heart, and dermis. We identified two independent IM subpopulations that are conserved across tissues: Lyve1 lo MHCII hi CX3CR1 hi (Lyve1 lo MHCII hi ) and Lyve1 hi MHCII lo CX3CR1 lo (Lyve1 hi MHCII lo ) monocyte-derived IMs, with distinct gene expression profiles, phenotypes, functions, and localizations. Using a new mouse model of inducible macrophage depletion (Slco2b1 flox/DTR ), we found that the absence of Lyve1 hi MHCII lo IMs exacerbated experimental lung fibrosis. Thus, we demonstrate that two independent populations of IMs coexist across tissues and exhibit conserved niche-dependent functional programming.

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U2 - 10.1126/science.aau0964

DO - 10.1126/science.aau0964

M3 - Article

C2 - 30872492

AN - SCOPUS:85062938159

SN - 0036-8075

VL - 363

JO - Science

JF - Science

IS - 6432

M1 - eaau0964

ER -