Two novel human and mouse DNA polymerases of the poIX family

Said Aoufouchi, Eric Flatter, Auriel Dahan, Ahmad Faili, Barbara Bertocci, Sebastien Storck, Frédéric Delbos, Laurentiu Cocea, Neetu Gupta, Jean Claude Weill, Claude Agnès Reynaud

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    Résumé

    We describe here two novel mouse and human DNA polymerases: one (pol λ) has homology with DNA polymerase β while the other one (pol μ) is closer to terminal deoxynucleotidyltransferase. However both have DNA polymerase activity in vitro and share similar structural organization, including a BRCT domain, helix-loop-helix DNA-binding motifs and polymerase X domain. mRNA expression of pol λ is highest in testis and fetal liver, while expression of pol μ is more lymphoid, with highest expression both in thymus and tonsillar B cells. An unusually large number of splice variants is observed for the pol μ gene, most of which affect the polymerase domain. Expression of mRNA of both polymerases is down-regulated upon treatment by DNA damaging agents (UV light, γ-rays or H202. This suggests that their biological function may differ from DNA translesion synthesis, for which several DNA polymerase activities have been recently described. Possible functions are discussed.

    langue originaleAnglais
    Pages (de - à)3684-3693
    Nombre de pages10
    journalNucleic Acids Research
    Volume28
    Numéro de publication18
    étatPublié - 15 sept. 2000

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