Unique genome replication mechanism of the archaeal virus AFV1

Mery Pina, Tamara Basta, Tessa E.F. Quax, Alexandra Joubert, Sonia Baconnais, Diego Cortez, Sarah Lambert, Eric Le Cam, Stephen D. Bell, Patrick Forterre, David Prangishvili

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    15 Citations (Scopus)

    Résumé

    The exceptional genomic content and genome organization of the Acidianus filamentous virus 1 (AFV1) that infects the hyperthermophilic archaeon Acidianus hospitalis suggest that this virus might exploit an unusual mechanism of genome replication. An analysis of replicative intermediates of the viral genome by two-dimensional (2D) agarose gel electrophoresis revealed that viral genome replication starts by the formation of a D-loop and proceeds via strand displacement replication. Characterization of replicative intermediates using dark-field electron microscopy, in combination with the 2D agarose gel electrophoresis data, suggests that recombination plays a key role in the termination of AFV1 genome replication through the formation of terminal loops. A terminal protein was found to be attached to the ends of the viral genome. The results allow us to postulate a model of genome replication that relies on recombination events for initiation and termination.

    langue originaleAnglais
    Pages (de - à)1313-1325
    Nombre de pages13
    journalMolecular Microbiology
    Volume92
    Numéro de publication6
    Les DOIs
    étatPublié - 1 janv. 2014

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