Unlocking bat immunology: Establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages

Peng Zhou; Yok Teng Chionh; Sergio Erdal Irac; Matae Ahn; Justin Han Jia Ng; Even Fossum; Bjarne Bogen; Florent Ginhoux; Aaron T. Irving; Charles Antoine Dutertre; Lin Fa Wang

doi:10.1038/srep38597

Unlocking bat immunology: Establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages

Peng Zhou, Yok Teng Chionh, Sergio Erdal Irac, Matae Ahn, Justin Han Jia Ng, Even Fossum, Bjarne Bogen, Florent Ginhoux, Aaron T. Irving, Charles Antoine Dutertre, Lin Fa Wang

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

31 Citations (Scopus)

Résumé

Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.

langue originale	Anglais
Numéro d'article	38597
journal	Scientific Reports
Volume	6
Les DOIs	https://doi.org/10.1038/srep38597
état	Publié - 9 déc. 2016
Modification externe	Oui

Accès au document

10.1038/srep38597

Autres fichiers et liens

Lien vers la publication dans Scopus

Contient cette citation

@article{b9d26764eb3c436e98e391fc7485510a,

title = "Unlocking bat immunology: Establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages",

abstract = "Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.",

author = "Peng Zhou and Chionh, {Yok Teng} and Irac, {Sergio Erdal} and Matae Ahn and {Jia Ng}, {Justin Han} and Even Fossum and Bjarne Bogen and Florent Ginhoux and Irving, {Aaron T.} and Dutertre, {Charles Antoine} and Wang, {Lin Fa}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2016.",

year = "2016",

month = dec,

day = "9",

doi = "10.1038/srep38597",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

}

TY - JOUR

T1 - Unlocking bat immunology

T2 - Establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages

AU - Zhou, Peng

AU - Chionh, Yok Teng

AU - Irac, Sergio Erdal

AU - Ahn, Matae

AU - Jia Ng, Justin Han

AU - Fossum, Even

AU - Bogen, Bjarne

AU - Ginhoux, Florent

AU - Irving, Aaron T.

AU - Dutertre, Charles Antoine

AU - Wang, Lin Fa

PY - 2016/12/9

Y1 - 2016/12/9

N2 - Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.

AB - Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.

UR - http://www.scopus.com/inward/record.url?scp=85006073407&partnerID=8YFLogxK

U2 - 10.1038/srep38597

DO - 10.1038/srep38597

M3 - Article

C2 - 27934903

AN - SCOPUS:85006073407

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 38597

ER -