@article{233ccf0641334ffcad867310358afcb9,
title = "Visual proteomics of the human pathogen Leptospira interrogans",
abstract = "Systems biology conceptualizes biological systems as dynamic networks of interacting elements, whereby functionally important properties are thought to emerge from the structure of such networks. Owing to the ubiquitous role of complexes of interacting proteins in biological systems, their subunit composition and temporal and spatial arrangement within the cell are of particular interest. 'Visual proteomics' attempts to localize individual macromolecular complexes inside of intact cells by template matching reference structures into cryo-electron tomograms. Here we combined quantitative mass spectrometry and cryo-electron tomography to detect, count and localize specific protein complexes in the cytoplasm of the human pathogen Leptospira interrogans. We describe a scoring function for visual proteomics and assess its performance and accuracy under realistic conditions. We discuss current and general limitations of the approach, as well as expected improvements in the future.",
author = "Martin Beck and Malmstr{\"o}m, {Johan A.} and Vinzenz Lange and Alexander Schmidt and Deutsch, {Eric W.} and Ruedi Aebersold",
note = "Funding Information: This project has been funded in part by ETH Zurich, the Swiss National Science Foundation (grant 31000-10767), federal funds from the US National Heart, Lung, and Blood Institute, the US National Institutes of Health (contract N01-HV-28179), by SystemsX.ch the Swiss initiative for systems biology, in part by the proteomics in time and space (PROSPECTS) European network of excellence, and with funds from the European Research Council project {\textquoteleft}Proteomics V3.0{\textquoteright}. M.B. was supported by a long-term fellowship of the European Molecular Biology Organization and a Marie Curie fellowship of the European Commission; J.A.M. was supported by a fellowship from the Swedish society for medical research (SSMF); and A.S. and V.L. were supported by the Competence Center for Systems Physiology and Metabolic Diseases. We thank O. Medalia, W. Baumeister, members of the electron microscopy facility of ETH Zurich (EMEZ) for continued support and F. F{\"o}rster for critical reading of the manuscript.",
year = "2009",
month = oct,
day = "19",
doi = "10.1038/nmeth.1390",
language = "English",
volume = "6",
pages = "817--823",
journal = "Nature Methods",
issn = "1548-7091",
number = "11",
}