Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate

Beomsue Kim; Masahiro Fukuda; Jung Yeol Lee; Dongdong Su; Srikanta Sanu; Aymeric Silvin; Audrey T.T. Khoo; Taejoon Kwon; Xiao Liu; Weijie Chi; Xiaogang Liu; Sejong Choi; Diana S.Y. Wan; Sung Jin Park; Jin Soo Kim; Florent Ginhoux; H. Shawn Je; Young Tae Chang

doi:10.1002/anie.201903058

Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate

Beomsue Kim, Masahiro Fukuda, Jung Yeol Lee, Dongdong Su, Srikanta Sanu, Aymeric Silvin, Audrey T.T. Khoo, Taejoon Kwon, Xiao Liu, Weijie Chi, Xiaogang Liu, Sejong Choi, Diana S.Y. Wan, Sung Jin Park, Jin Soo Kim, Florent Ginhoux, H. Shawn Je, Young Tae Chang

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

24 Citations (Scopus)

Résumé

Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure–activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.

langue originale	Anglais
Pages (de - à)	7972-7976
Nombre de pages	5
journal	Angewandte Chemie - International Edition
Volume	58
Numéro de publication	24
Les DOIs	https://doi.org/10.1002/anie.201903058
état	Publié - 11 juin 2019
Modification externe	Oui

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10.1002/anie.201903058

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Kim, B., Fukuda, M., Lee, J. Y., Su, D., Sanu, S., Silvin, A., Khoo, A. T. T., Kwon, T., Liu, X., Chi, W., Liu, X., Choi, S., Wan, D. S. Y., Park, S. J., Kim, J. S., Ginhoux, F., Je, H. S., & Chang, Y. T. (2019). Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate. Angewandte Chemie - International Edition, 58(24), 7972-7976. https://doi.org/10.1002/anie.201903058

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title = "Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate",

abstract = "Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure–activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.",

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author = "Beomsue Kim and Masahiro Fukuda and Lee, {Jung Yeol} and Dongdong Su and Srikanta Sanu and Aymeric Silvin and Khoo, {Audrey T.T.} and Taejoon Kwon and Xiao Liu and Weijie Chi and Xiaogang Liu and Sejong Choi and Wan, {Diana S.Y.} and Park, {Sung Jin} and Kim, {Jin Soo} and Florent Ginhoux and Je, {H. Shawn} and Chang, {Young Tae}",

note = "Publisher Copyright: {\textcopyright} 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2019",

month = jun,

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doi = "10.1002/anie.201903058",

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AU - Fukuda, Masahiro

AU - Lee, Jung Yeol

AU - Su, Dongdong

AU - Sanu, Srikanta

AU - Silvin, Aymeric

AU - Khoo, Audrey T.T.

AU - Kwon, Taejoon

AU - Liu, Xiao

AU - Chi, Weijie

AU - Liu, Xiaogang

AU - Choi, Sejong

AU - Wan, Diana S.Y.

AU - Park, Sung Jin

AU - Kim, Jin Soo

AU - Ginhoux, Florent

AU - Je, H. Shawn

AU - Chang, Young Tae

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