YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules

Konstantin G. Chernov, Alain Mechulam, Nadezhda V. Popova, David Pastre, Elena S. Nadezhdina, Olga V. Skabkina, Nina A. Shanina, Victor D. Vasiliev, Anne Tarrade, Judith Melki, Vandana Joshi, Sonia Baconnais, Flavio Toma, Lev P. Ovchinnikov, Patrick A. Curmi

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

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    Résumé

    Background. YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs. Results. We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes. Conclusion. These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation.

    langue originaleAnglais
    Numéro d'article23
    journalBMC Biochemistry
    Volume9
    Numéro de publication1
    Les DOIs
    étatPublié - 1 janv. 2008

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